Part:BBa_K1810001
Repeating QSP Chain with HlyA secretion Tag
QSP is a tripeptide drug that can inhibit the glucose reuptake pathway of the SGLT1 and SGLT2 membrane proteins of the Kidneys. This sequence codes for a repeated sequence of three sets of QSP. The string of tripeptides is fused with the HlyA secretion tag, which can be used in tandem with the HlyB and HlyD membrane proteins to secret the desired protein to the extracellular space of an E. coli cell. Once outside the cell, the string of peptides was to be cleaved by a proline iminopeptidase that is bound on the membrane of the E. coli cell, and thus ultimately produces the pharmacologically-active tripeptides. The coding sequence is placed under the OmpC promoter; this promoter is activated by phosphorylated OmpR, the concentration of which increases as extracellular osmolarity increased. Therefore, this system can be induced under conditions of high glucose in the cell environment, and therefore work to lower the level of glucose in the body acting through the kidneys.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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